Cell-Free Sampling Probes
Applications
Microbial and Mammalian Sampling
FISP® will perform well whether the process is a microbial fermentation requiring multiple samples per hour or a mammalian cell culture requiring less frequent sampling. Sampling with FISP® provides an excellent means of obtaining material for determining glucose, lactate, glutamine, glutamate and ammonia levels in perfused mammalian cell cultures. This data can, in turn, be used to adjust the perfusion rate and to determine the optimal time for infection and harvest.
The following information has been generated by end users that have utilized
the FISP® for a minimum of one year. The majority of these results are from processes that have
been in place for 2 or more years. Several of the following have gone through GMP validation.
| Organism |
Fermentation Period (hours) |
Sample Flow(ml/min) |
| Bacteria |
|
|
| Bacillus lichenif |
36 |
1.2 |
| Bacillus subtilis |
20 |
2.0 |
| E. Coli K12 |
16 |
1.0 |
| E. Coli K12 |
24 to 72 |
0.75 |
| E. Coli K12 |
28 |
2.0 |
| E. Coli K12 |
13 |
1.5 |
| E. Coli K12 |
18 |
2.0 |
| E. Coli Recomb. |
12 |
1.0 |
| E. Coli Recomb. |
40 |
1.5 |
| E. Coli Recomb. |
36 |
1.5 |
| Mycobacter |
45 |
1.0 |
| Strepto coccus Pheu. |
9 |
1.0 |
| Yeast |
|
|
| Sacch. Cerevisiae |
48 |
1.0 |
| Sacch. Cerevisiae |
260 |
0.8 |
| Sacch. Cerevisiae |
120 |
1.0 |
| Sacch. Cerevisiae |
24 to 150 |
1.0 |
| Fungal |
|
|
| Acremonium chrysogenum |
160 |
1.0 |
| Aspergillus awamori |
180 |
1.0 |
| Aspergillus niger |
190 |
1.0 |
| Cephalosporium ac. |
200 |
1.0 |
| Penicillium chrysogenum |
200 |
0.7 |
| Spodoptera frugi |
200 |
1.5 |
| Spodoptera frugi |
280 |
1.0 |
| Spodoptera frugi |
200 |
0.8 |
| Cell Culture |
|
|
| BHK 21, CHO |
42 (Days) |
0.5 |
| Mouse/Mouse hybrid |
120 |
0.4 |
| Mouse/Rat hybrid |
41 (Days) |
0.4 |
| NSO |
37 (Days) |
0.75 |
