Cell-Free Sampling Probes
Sterile In-situ Sampling System for Fermentation and Cell Culture
Flownamics® has developed the FISP®, which is a sampling probe capable of withdrawing sterile,
cell-free samples from fermentors and bioreactors. FISP® allows direct on-line sample transfer to a
variety of analyzers, such as biochemistry and HPLC systems, as well as collection for off-line analysis.
FISP® is a small, tube-shaped, sterilizable 316 stainless steel carrier which is surrounded by a tubular,
micro-porous membrane. They are available for 12, 19 and 25 mm ports; for use in laboratory, pilot or
industrial scale vessels. FISP® is widely utilized in the fermentation and cell culture fields since
it's introduction to the market in 1996.
Features of the FISP®
- Sterile, cell-free sampling for off-line and on-line analysis
- FISP® Probes can be autoclaved or sterilized in-place
- Used for aerobic or anaerobic bacterial cultures, as well as yeast, fungi, algae, insect, animal and mammalian cell cultures
- Risk free sampling due to elimination of bypass filters, which circulates material back into vessel
- Accommodates small laboratory vessels up to large full-scale production vessels with 12, 19 and 25 mm models
- Easy to use and maintain; no operator training required
- Interfaces the fermentor or bioreactor with a variety of analytical systems
- Biochemistry Analyzers
- Fraction collectors
- Autosamplers
- Flow Injection Analysis (FIA)
- HPLC/UPLC
- Design minimizes dead volume, which allows for consistent and accurate sampling, and reduces probe flush time
- Durable in design and construction to withstand the temperatures, pressures, viscosities, shear forces and chemicals typical of fermentation environments
Materials
- Probes and fittings are 316 stainless steel
- O-rings are Ethylene Propylene (EPDM)
- Membrane material is ceramic
Features of the FISP®
