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New Product: Seg-Flow S3 Automated On-Line Sampling System

Introducing the next generation of the Seg-Flow Automated Sampling System, the Seg-Flow S3, featuring...

  • Smart Flow Control
  • Improved Ease of Use
  • Optimized Operator Interface
  • Increased Reliability

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Advantages of Deploying PI for the Collection of Discrete Data from the Seg-Flow Automated Sampling System

Kristin O'Neill, Associate Principal Scientist in Merck & Co.'s Biologics Process Research and Development (BPRD) group (Kenilworth, NJ) presented details related to their use of the Seg-Flow as a presenter at PI World in San Francisco, California, in April, 2019. Merck is an OSIsoft EA customer with PI deployed around the globe in research and manufacturing environments.

Historically within Biologics Process Research and Development (BPRD), PI has been used heavily to capture continuous time series data from bioreactor controllers at multiple scales. A recent addition of Flownamics’ fully automated system for bioreactor sampling, analysis, and feed control provided an opportunity to integrate a substantial amount of discrete sample data into the PI system. This innovative use case enabled remote monitoring of all aspects of the cell culture process as well as provided a tool to monitor the system health of the auotsampler itself.

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Flownamics Sponsored Seminars Announced

Amid an undeniably rapidly evolving bioprocessing environment, driven in no small part by challenges that include integration with single-use solutions, increasingly scaled down applications, emergence of continuous processing and more, the need for access to knowledge and information is greater than ever.

Please join us for one of our one-day seminars by bioprocessing professionals, end users and suppliers alike, discussing the challenges associated with bioprocess optimization and the latest proven solutions being deployed to address these challenges. Speakers will include subject matter experts from our seminar partners addressing applications upstream and downstream alike. Our speakers will be sharing novel insights, case studies and taking questions on specific challenges and sharing suggested solutions.

February 20 | Emeryville, CA and February 28 | Cambridge, MA


Flownamics featured on the cover of Genetic Engineering & Biotechnology News (GEN) on April 15, 2018

At the Optimizing Cell Culture Technology conference, Flownamics gave a presentation that illustrated how its Seg-Flow® automated platform for sampling and feed control can help optimize process conditions and performance, improving efficiency and minimizing the risk of human error. The Seg-Flow sampling system uses Segmented Online Sampling™ technology to achieve accurate and rapid sampling from up to eight vessels or process streams, with delivery of samples to as many as four analyzers...

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New Products Shipped in 2018 Have Ultrasonic Sensors

Starting at the beginning of 2018, all new Seg-Flow®’s, Sample-Mod™’s, Seg-Mod™’s, and cables sold will have ultrasonic sensors, which measure distance by sending out a sound wave at a specific frequency and listening for that sound wave to bounce back. Existing products, the Seg-Flow® 1200’s, 1200 Plus’s, and 4800’s, and Seg-Mod™’s, can be upgraded to ultrasonic sensors at the prices shown on the Flownamics’ 2018 pricelists. Sample-Mods™ cannot be upgraded.

Contact Us if you want to upgrade your existing products.


New Product: Interface Kit for Kaiser Optical Systems Raman Probe

Our Seg-Flow® Online Sampling System can now be paired with the industry leading RamanRxn2™ Multi-Channel Analyzer manufactured by Kaiser Optical Systems (Ann Arbor, MI). We’re excited to introduce this new sample interface kit.

The Raman Rxn2™ can measure glucose, glutamine, glutamate, lactate, ammonium, osmolality, viability, viable cell density, and total cell density.

This new Sampling Interface Kit (SIK) allows the Seg-Flow® 1200 Plus or 4800 to integrate to a single RamanRxn2™ probe and connect this probe to a maximum of 8 bioreactors, providing a cost-saving alternative to using 8 individual Raman probes in a customer's process.

This interface is compatible with the Seg-Flow® 1200 Plus and the 4800 and requires Flow-Web™ OPC Software. The  Sample Interface Kit for Kaiser Optical System Raman Probe part number is: A-SIK-14.

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Contact Us if you have questions about this new interface.


New Product: FlowFraction™ 500

Flownamics shipped its first FlowFraction™ 500 model which has the ability to hold four Peltier Racks. The FlowFraction™ is used with our Seg-Flow® Automated On-Line Sampling Solution for on-line fraction collection from up to eight vessels for further off-line analysis of your bioprocess. The FlowFraction™ system is customizable to accommodate a variety of tube types and sizes, septum piercing, and temperature control. Reagent addition and sample dilution may be performed prior to vial deposit with the optional Seg-Flow® Sample Module. The Flow-Web™ OPC Software controls and monitors all sample collection functions. Internal flow paths are self cleaning after every sample cycle, ensuring no sample carryover or contamination.

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Contact Us to upgrade to the FlowFraction™ 500 Fraction Collector.


Enoil Bioenergies Research Laboratories

Research on biofuels is one of the most dynamic areas in biotechnology today. Enoil Bioenergies Research Laboratories (EBERL), a biotechnology and renewable energy company registered in Switzerland, strives to provide the fundamental science to underpin a cost-effective, advanced biofuels industry.

Using systems biology approaches, they are focusing on new strategies to reduce the impact of key cost-driving processes in the overall production of cost-competitive biofuels from biomass. For these biofuels to be adopted on a large scale, they must represent environmentally sustainable and economically competitive alternatives to existing fuel systems. New strategies and findings emanating from the centres’ fundamental research are addressing challenges for cost-effective advanced biofuels production.

They state the power of coupling parallel bioreactors and Seg-Flow® automated on-line sampling technologies:

  • Has enabled research staff to implement remote-controlled, automated process trigger sampling as an integral part of its yeast strain characterization activities.
  • Can significantly reduce project timelines and increase the efficiency of the microbial strain characterization and selection process.
  • Is better able to rapidly characterize process events, parameters, and stress responses that impact yeast strain gene regulation and, ultimately, biofuel production.

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Eppendorf Webinar: Automated Bioreactor Sampling and Glucose Control

Industry leader Eppendorf (Hamburg, Germany) hosted a webinar which featured Flownamics' Seg-Flow® Automated On-Line Sampling Solution. Eppendorf scientist, Dr. Stacey Willard, shares valuable insights on streamlining bioprocess development efforts through automation.

Manual bioreactor sampling and feeding can be a costly endeavor, both in terms of labor costs and the increased risk for contamination each time the sterile boundary is penetrated. Automated sampling and glucose control can accelerate biopharmaceutical development and decrease costs and time-to-market.

In this webinar, a solution for automated glucose monitoring and feeding during a bioprocess run is presented. They integrated an autosampler and an analyzer with the bioprocess control and were able to completely automate glucose control. The design of the automation program is fully customizable, allowing the user to implement their own control strategy with custom nutrient target concentration, custom sampling frequencies, etc.

Most importantly, the sterile envelope is not penetrated for each sample and feed since the sample is acquired and delivered to the analyzer without human intervention. Following sample analysis, the data is transmitted to the SCADA software where the feed program triggers a pump to deliver the appropriate dose of glucose to the growing culture. Using this strategy, glucose and other nutrients can be maintained at a more stable concentration during critical portions of a cell culture process which may have an impact on cell and product yields.

Learning Objectives

  • Learn how to set up automated nutrient monitoring and feeding.
  • Understand the basics of programming in order to create feedback loops.

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Nova Biomedical Educational Webinar: Bioprocess Automation for PAT with BioProfile® FLEX 2 Plus Seg-Flow®

Our newest integration, the BioProfile® FLEX 2, was featured in a webinar hosted by Nova Biomedical. Nova Biomedical (Waltham, MA) develops and manufactures automated chemistry and cell culture analyzers to rapidly test key nutrients, metabolites, gases, osmolality, and cell density for mammalian cell culture and fermentation media. This webinar discusses an online automation solution for cell culture sampling and comprehensive analysis for bench-scale bioreactor cultures. Nova’s BioProfile FLEX 2, with 16 key cell culture tests and maintenance-free sensors, can now be paired with Flownamics’ Seg-Flow® online auto-sampler, enabling completely automated online sampling from up to eight bioreactor cultures.

Learning Objectives

  • Learn about FLEX 2’s innovative design that provides comprehensive analysis of 16 key cell culture chemistries.
  • Discover how the collaboration between Flownamics and Nova Biomedical allows off-line and at-line data to seamlessly integrate into a multifunctional online PAT tool through Seg-Flow®’s communication interface.
  • See how FLEX 2 automation through the Seg-Flow® interface reduces labor, saves costs, and provides the highest quality data possible.
  • Explore real-world examples of automated analysis of micro-bioreactor cell cultures.
  • Review the advantages of FLEX 2’s small sample volume and unique modular design.
  • Gain insight into the advantages of FLEX 2’s maintenance-free MicroSensor Card™ technology.

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New Product: Seg-Mod™ 202

Seg-Mod™ 202 Features / Benefits

  • Ultrasonic sensors offer even better pinpoint accuracy and proven reliability over infrared sensors
  • Patent pending dual fluid sensor technology ensures accurate measure of flow rates and features a disposable fluid path
  • Calculation of fluidic pull for accurate cell-free sampling means when viscosity increases, flowrate decreases
  • Self-calibrating fluidic module is ideal for high cell density cultures
  • Self-calibration of fluidic pull provides for accurate sample withdrawal
  • Precise fluid management system for vessel sampling operations
  • Scale independent, can be used with laboratory, pilot, or production vessels
  • Compatible with any manufacturer or vessel design
  • Mounts easily on FISP® probes

This new product has been added to our website’s Accessories page.

Contact Us to purchase the new Seg-Mod™ 202.


New Product: Sample-Mod™ 300

Sample-Mod™ 300 Features / Benefits

  • New pinch valves
  • More accurate
  • Higher precision
  • Zero dead volume

To upgrade from an earlier version of the Sample-Mod™, a customer would need (1) either a Seg-Flow® 1200 Plus or 4800 Sampling System with an ultrasonic sensor and version E connector board; (2) Seg-Mod™(s) with ultrasonic sensors; (3) ultrasonic sensor cable; and (4) software upgrade.

This new product has been added to our website’s Accessories page.

Contact Us to purchase the new Sample-Mod™ 300.


Research: A Novel Approach to Real Time Monitoring of Antibody Titer in a Fed-Batch Bioreactor Run

Aragen Bioscience (Morgan Hill, CA) presented the results of their research as a poster at the BioProcessing Summit 2017, held in Boston, MA, which utilized a Seg-Flow® Automated Sampling System connected to a real-time antibody titer device.

Abstract: In most cases, the titer of an antibody being expressed in a stirred bioreactor is measured offline. The process involves removing an aliquot aseptically from the bioreactor, centrifuging the aliquot and analyzing the resulting supernatant. Such an approach may limit throughput, workflow and efficiency. Aragen is evaluating a prototype chromatography-based device that is directly connected to a bioreactor.

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Research: Enhancing Automated Sampling, Process Monitoring, and Nutrient Feedback Control for a System of 3-L Bioreactors

The Bioprocess Development team at Merck and Co, Inc. (Kenilworth, NJ), presented the results of their research as a poster at ESACT 2017, held in Lausanne, Switzerland, which utilized two Seg-Flow® Online Sampling Systems, a Sample-Mod™, interfaced with a Roche Cedex HiRes Cell Analyzer, Roche Cedex Bio HT Analyzer, and Nova Biomedical BioProfile FLEX Analyzer.

Abstract: Key to effective upstream development is the presence of resource-efficient systems for examining complex process options. In this presentation, we report on the progress of a completely automated sampling and feed control system capable of simultaneously evaluating different metabolic-based feed strategies for bench-scale cell culture processes.

Though there are automated feed systems available for microscale vessels, to date there is not a complete 3-L scale sample and feed automation solution as described here, with capability to transfer samples from multiple bioreactors (single-use or glass) to a suite of independent analyzers with concurrent feedback control of nutrient feed pumps.

There are multiple drivers for developing automated systems for benchtop bioreactors, ranging from increased operating efficiency to enhancing the toolbox for process development. Deeper process understanding obtained through examining metabolic-tailored feeding strategies, is essential to the development of state-of-the-art upstream processes with emphasis on product quality attributes.

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Research: Automated Sampling Enables Rapid NIRS Calibration Model Construction

Jim Collett of the U.S. Department of Energy (DOE), Pacific Northwest National Laboratory, Bioenergy Technologies Office (BETO), credits one of the critical success factors of the project, "Advanced Supervisory Control and Data Acquisition (SCADA) for Biochemical Process Integration," as achieving real-time tracking and control of critical process parameters (CPPs), which used a Flownamics Seg-Flow® 8000i Automated Sampling Solution, Seg-Mod™ Segmented On-Line Sampling, and FlowFraction™, with a YSI 2950 Biochemical Analyzer.

Their challenge was to facilitate fed-batch bioconversion of highly variable biomass feedstocks which would require advanced supervisory control and data acquisition (SCADA) systems for cost-competitive production of hydrocarbon biofuels and chemicals with the goal of working with industrial partners on technology to market transfer of Process Analytical Technologies (PAT) for biorefinery SCADA systems to improve process control, reduce manual sampling, and increase product quality.

The outcome would be that bioenergy startups and established biorefineries would have access to proven PAT equipment and methods that integrate seamlessly with SCADA systems to enable profitable scale-up and informative scale-down of industrial bioprocesses.

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Article: Automated Collection and Analysis of Bioreactor Samples to Enable Quality by Design Initiatives

As biomanufacturing processes become increasingly complex, the FDA has stressed the importance of their Quality by Design (QbD) and Process Analytical Technology (PAT) initiatives in an effort to encourage more consistent manufacturing processes and product quality. A QbD approach provides a systematic look at bioprocess development, which attempts to predict unique challenges and address them in advance. A key aspect of QbD is defining critical quality attributes (CQA) and critical process parameters (CPP) and then monitoring these closely through the use of PAT. However, a challenge in this approach is to ensure that the culture is being monitored precisely, so that critical process parameters can be maintained accurately.

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Report: Global $23.99 Billion Cell Expansion Market 2016 - Forecast to 2022

Research and Markets has announced the addition of the "Cell Expansion Global Market - Forecast to 2022" report to their offerings. The report states that the cell expansion market is expected to grow at a double digit compound annual growth rate (CAGR) to reach $23,997 million by 2022 and that Flownamics is a key player of the cell expansion market.

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New Product: Flow-Web™ OPC Controller

The Flow-Web™ OPC Controller improves bioprocess technology and control by expanding Process Analytical Technology options and enhancing existing SCADA system functionality. The Flow-Web™ OPC Controller seamlessly acquires and integrates data from multiple on-line, at-line and off-line analytical instruments and exports the integrated data into any OPC-enabled SCADA system or data historian. Flow-Web™ OPC can also remotely control some external devices. The Flow-Web™ OPC Controller can communicate to up to 3 instruments through RS-232 serial communication and / or up to 8 instruments via TCP / IP communications. All system functions are controlled by the Flow-Web™ software which can be accessed remotely using a web browser.

Flow-Web™ OPC Controller Benefits

  • Data acquisition and analysis integration
  • Enhance process monitoring through integrated analytical approach
  • Improve process control and performance
  • 21 CFR Part 11 compliant

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New Product: PMR™ Controller

Our new PMR™ Controller features patent pending dual fluid sensor technology which offers the ideal control solution for your perfusion process.

Addition and removal pumps are housed together in one main unit along with two dual fluid sensor flow meters which are used to auto-calibrate the media addition and filtrate removal pumps at user defined intervals. Multiple units can be controlled by one SCADA PC to provide easy parallel perfusion control. The small footprint PMR™ Controller eliminates the need for bulky scales prone to environmental error.

The system is designed to work with your existing perfusion set-up and uses gamma radiated disposable tubing that can be welded directly into your fluid path.

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Distributor Highlight: Broadly-James LTD

Broadley-James LTD distributes and supports Flownamics Seg-Flow® Automated Sampling Systems in the United Kingdom. Their staff is fully trained and qualified to provide customers in the UK with sales and service for all Seg-Flow® products. In the video below, David Price, Broadley-James LTD Sales Manager, demonstrates Flownamic’s Seg-Flow® Automated Sampling System at KNect 365 at Life Sciences BioProduction Congress Meeting in Dublin, Ireland (October 19-20, 2016). David Price outlines the features and benefits of the Seg-Flow® Automated Sampling System, along with other equipment manufactured and sold by Broadley James.

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